Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00145258-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00145258-M01, RRID:AB_608654
- Product name
- Mouse Monoclonal Goosecoid Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GSC - goosecoid
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Generation of pluripotent stem cells from adult human testis.
Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Bühring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
Nature 2008 Nov 20;456(7220):344-9
Nature 2008 Nov 20;456(7220):344-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Goosecoid Antibody (4H7) [H00145258-M01] - Analysis of GSC expression in transfected 293T cell line by GSC monoclonal antibody (M01), clone 4H7.Lane 1: GSC transfected lysate(28.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Goosecoid Antibody (4H7) [H00145258-M01] - Detection limit for recombinant GST tagged GSC is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Goosecoid Antibody (4H7) [H00145258-M01] - Analysis of GSC transfected lysate using anti-GSC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GSC MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Goosecoid Antibody (4H7) [H00145258-M01] - Analysis of monoclonal antibody to GSC on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.