Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF4086 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Goosecoid Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Goosecoid in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P56915
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids.
Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip.
Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System.
Yoney A, Etoc F, Ruzo A, Carroll T, Metzger JJ, Martyn I, Li S, Kirst C, Siggia ED, Brivanlou AH
eLife 2018 Oct 12;7
eLife 2018 Oct 12;7
Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip.
Musah S, Dimitrakakis N, Camacho DM, Church GM, Ingber DE
Nature protocols 2018 Jul;13(7):1662-1685
Nature protocols 2018 Jul;13(7):1662-1685
Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System.
Low K, Wong LY, Maldonado M, Manjunath C, Horner CB, Perez M, Myung NV, Nam J
Stem cell reports 2017 May 9;8(5):1329-1339
Stem cell reports 2017 May 9;8(5):1329-1339
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Goosecoid in BG01V Human Embryonic Stem Cells. Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.