PA5-120373

antibody from Invitrogen Antibodies

Targeting: DNER bet, UNQ26

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120373

Provider

Invitrogen Antibodies

Product name

DNER Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: Hela, HT-29, Mouse brain, Mouse eye, Mouse small intestine, Rat brain. The target is usually found in the following locations: Cell membrane, Single-pass type I membrane protein. Immunogen sequence: PATGWTESMA PRQLQPVPAT QEPDKILPRS QATVTLPTWQ PKTGQKVVEM KWDQVEVIPD IACGNASSNS SAGGRLVSFE VPQNTSVKIR QDATASLILL W

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.56 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DNER in various cell lines using a DNER Polyclonal antibody (Product # PA5-120373) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DNER in L929 cells using a DNER Polyclonal antibody (Product # PA5-120373). Blue: DAPI for nuclear staining.