H00168433-M01

antibody from Abnova Corporation

Targeting: RNF133

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00168433-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00168433-M01, RRID:AB_519022

Product name

RNF133 monoclonal antibody (M01), clone 3D6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RNF133.

Antigen sequence

FFHKNCIDPWILPHGTCPICKCDILKVLGIQVVVE
NGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTS
DKVIHVEENPTSQNNDIQPHSVVEDVHPSP


Isotype

IgG

Antibody clone number

3D6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RNF133 expression in transfected 293T cell line by RNF133 monoclonal antibody (M01), clone 3D6.Lane 1: RNF133 transfected lysate(42.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNF133 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol