Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-31044 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF133 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562. Predicted reactivity: Rhesus Monkey (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RNF133 Polyclonal Antibody (Product # PA5-31044). Sample (30 µg of whole cell lysate). Lane A: K562. 10% SDS PAGE. RNF133 Polyclonal Antibody (Product # PA5-31044) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RNF133 (Product # PA5-31044) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNF133 Polyclonal Antibody detects RNF133 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. RNF133 Polyclonal Antibody (Product # PA5-31044) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNF133 Polyclonal Antibody detects RNF133 protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. RNF133 Polyclonal Antibody (Product # PA5-31044) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.