PA5-46945

antibody from Invitrogen Antibodies

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Recommended by provider)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46945

Provider

Invitrogen Antibodies

Product name

TNF alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In sandwich ELISAs, approximately 50% cross-reactivity with recombinant rat TNF- alpha is observed and less than 1% cross-reactivity with recombinant human TNF-alpha is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Seed-induced Aβ deposition alters neuronal function and impairs olfaction in a mouse model of Alzheimer's disease.

Ziegler-Waldkirch S, Friesen M, Loreth D, Sauer JF, Kemna S, Hilse A, Erny D, Helm C, D Errico P, Prinz M, Bartos M, Meyer-Luehmann M
Molecular psychiatry 2022 Oct;27(10):4274-4284

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Reduction of the RNA Binding Protein TIA1 Exacerbates Neuroinflammation in Tauopathy.

LeBlang CJ, Medalla M, Nicoletti NW, Hays EC, Zhao J, Shattuck J, Cruz AL, Wolozin B, Luebke JI
Frontiers in neuroscience 2020;14:285

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TNF alpha in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TNF alpha in immersion fixed activated mouse T Cells. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 15 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TNF alpha in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TNF alpha in immersion fixed activated mouse T Cells. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 15 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of mouse TNF-a is shown by increase in RFU (relative fluorescent units - measure of decrease in cytotoxicity) with increasing concentrations of TNF-a polyclonal antibody (PA5-46945).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of TNF alpha in L‚929 mouse fibroblast cell line. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945). Recombinant Mouse TNF‚α induces cytotoxicity in the L‚929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‚α (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF‚α Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 Reduction of TIA1 increases production of TNFalpha in tauopathy. (A) log 2 fold change of TNFalpha expression in WT TIA1 +/+ and P301S subjects that are TIA1 +/+ , TIA1 +/- , and TIA1 -/- at 5 months (light gray) and 9 months (dark gray). n = 3 animals/genotype (B) Percent area occupied by TNFalpha particles in (C) . 5 months (light gray), 9 months (dark gray). n = 9-12 fields, from 3-4 animals/genotype. (C) High resolution (40x) confocal image stacks depicting the apex and adjacent dentate gyrus including the dorsal and ventral dentate granule cell layers and hilus in P301S subjects that are TIA1 +/+ , TIA1 +/- and TIA1 -/- at 5 months (top) and 9 months (bottom). TNFalpha visualized in purple. Scale = 25 mum. All statistical comparisons calculated with One-Way ANOVA with Bonferroni post hoc test, * p