21445-1-AP

antibody from Proteintech Group

Targeting: DDRGK1 C20orf116, dJ1187M17.3, UFBP1

Provider product page for 21445-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [49]
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Validations
Western blot [1]

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Product number: 21445-1-AP - Provider product page
Provider: Proteintech Group
Product name: DDRGK1 antibody
Antibody type: Polyclonal
Description: KD/KO validated DDRGK1 antibody (Cat. #21445-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DDRGK1 protein structure - 21445-1-AP shown in red.

Submitted references The coordinated action of UFMylation and the RQC pathways clears arrested polypeptides at the ER.

Mihailovic M, Anisimova AS, Erte B, Zhan N, Styliara I, Dagdas Y, Karagöz GE
The EMBO journal 2026 May;45(9):3252-3275

DysUFMylation reprograms immunosuppressive neutrophils to potentiate anti-PD-1 therapy in hepatocellular carcinoma.

Gao X, Xu M, Han Z, Hu Z, Su Y, Sun G, Chen W, Qiu S, Qiu S, Hung MC, Zhou C, Yi Y
Cancer letters 2026 May 1;645:218395

XIAP Stabilizes DDRGK1 to Promote ER-Phagy and Protects Against Noise-Induced Hearing Loss.

Yan L, Zhang Y, Du J, Zhu Y, Cao W, Wei Y, Wu H, Qiu S, Pan S, Chen L, Liang P, Chai R, Yang J, Fang Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar;13(18):e11217

Targeting the UFL1-AKT cascade suppresses triple-negative breast cancer progression.

Yang X, Wen Y, Ma X, Qin S, Liu Y, Chen J, Zhang H, Goding CR, Cui R, Liu T
Nature communications 2026 Jan 20;17(1):613

MCM5 UFMylation regulates replication origin firing and fork progression.

Li Z, Wu X, Liu L, Rao S, Liao Y, Liu M, Peng B, Zhang Q, Xia Y, Zhai Y, Takeda S, Xu X
The EMBO journal 2025 Nov;44(21):6019-6050

DysUFMylation of SREBP1 Promotes the Progression of Hepatocellular Carcinoma by Reprogramming Lipid Metabolism.

Gao X, Han Z, Xu M, Wang Z, Sun G, Xiao H, Zhang D, Qiu S, Ren N, Zhou C, Yi Y
Journal of clinical and translational hepatology 2025 Nov 28;13(11):949-963

UFMylation safeguards human hepatocyte differentiation and liver homeostasis by regulating ribosome dissociation.

Yang S, Wang L, Gao R, Li Y, Zhang D, Wang C, Liu G, Na J, Xu P, Wang X, Jia Y, Huang Y
Cell reports 2025 May 27;44(5):115686

UFMylation promotes orthoflavivirus infectious particle production.

Schmidt HM, Sorensen GC, Lanahan MR, Bland KM, Aufgebauer CJ, Park M, Grabowski J, Horner SM
Journal of virology 2025 Jul 22;99(7):e0065425

UFMylation promotes orthoflavivirus infectious particle production.

Schmidt HM, Sorensen GC, Lanahan MR, Grabowski J, Park M, Horner SM
bioRxiv : the preprint server for biology 2025 Jan 9;

DDRGK1 preserves intervertebral disc development through ufmylation.

Lu M, Zhou T, Yang X, Liu K, Rong K, Ma P, Qin A, Zhao J
Cellular and molecular life sciences : CMLS 2025 Dec 29;83(1):47

Akt-phosphorylated UFL1 UFMylates ArpC4 to promote metastasis.

Zhao K, Hu H, Fang D, Xie M, Chen J, Zhang S, Tang S, Wu M, Guo X, Yu N, Yao B, Jiang W, Wang C, Mei Y
Nature structural & molecular biology 2025 Aug;32(8):1528-1541

UFBP1 Ameliorates Heat Stress-Induced Apoptosis via Mitochondria-Mediated Pathway in Bovine Mammary Epithelial Cells.

Li Y, Yu R, Tan S, Jiang Y, Sun L, Shen M, Zhang C, Chen K, Li C
Animals : an open access journal from MDPI 2025 Apr 27;15(9)

Protein UFMylation regulates early events during ribosomal DNA-damage response.

Panichnantakul P, Aguilar LC, Daynard E, Guest M, Peters C, Vogel J, Oeffinger M
Cell reports 2024 Sep 24;43(9):114738

The UFMylation pathway is impaired in Alzheimer's disease.

Yan T, Heckman MG, Craver EC, Liu CC, Rawlinson BD, Wang X, Murray ME, Dickson DW, Ertekin-Taner N, Lou Z, Bu G, Springer W, Fiesel FC
bioRxiv : the preprint server for biology 2024 May 26;

UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER.

DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wąchalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature 2024 Mar;627(8003):445-452

UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity.

He C, Xing X, Chen HY, Gao M, Shi J, Xiang B, Xiao X, Sun Y, Yu H, Xu G, Yao Y, Xie Z, Xing Y, Budiarto BR, Chen SY, Gao Y, Lee YR, Zhang J
Molecular cell 2024 Mar 21;84(6):1120-1138.e8

The ufmylation cascade controls COPII recruitment, anterograde transport, and sorting of nascent GPCRs at ER.

Xu X, Huang W, Bryant CN, Dong Z, Li H, Wu G
Science advances 2024 Jun 21;10(25):eadm9216

CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma.

Zhuo R, Zhang Z, Chen Y, Li G, Du S, Guo X, Yang R, Tao Y, Li X, Fang F, Xie Y, Wu D, Yang Y, Yang C, Yin H, Qian G, Wang H, Yu J, Jia S, Zhu F, Feng C, Wang J, Xu Y, Li Z, Shi L, Wang X, Pan J, Wang J
Cancer letters 2024 Jun 1;591:216882

Eg5 UFMylation promotes spindle organization during mitosis.

Li G, Huang Y, Han W, Wei L, Huang H, Zhu Y, Xiao Q, Wang Z, Huang W, Duan R
Cell death & disease 2024 Jul 31;15(7):544

DDRGK1-mediated ER-phagy attenuates acute kidney injury through ER-stress and apoptosis.

Jin H, Yang Y, Zhu X, Zhou Y, Xu Y, Li J, Qi C, Shao X, Wu J, Wu S, Cai H, Gu L, Mou S, Ni Z, Li S, Lin Q
Cell death & disease 2024 Jan 17;15(1):63

Endoplasmic reticulum stress of endometrial mesenchymal stem cells in endometriosis.

Rencber SF, Yazır Y, Sarıhan M, Sezer Z, Korun ZEU, Ozturk A, Duruksu G, Guzel E, Akpınar G, Corakci A
Tissue & cell 2024 Dec;91:102544

Mithramycin targets head and neck cancer stem cells by inhibiting Sp1 and UFMylation.

Derfi KV, Vasiljevic T, Dragicevic T, Glavan TM
Cancer cell international 2024 Dec 19;24(1):412

The UFMylation pathway is impaired in Alzheimer's disease.

Yan T, Heckman MG, Craver EC, Liu CC, Rawlinson BD, Wang X, Murray ME, Dickson DW, Ertekin-Taner N, Lou Z, Bu G, Springer W, Fiesel FC
Molecular neurodegeneration 2024 Dec 18;19(1):97

Loss of DDRGK1 impairs IRE1α UFMylation in spondyloepiphyseal dysplasia.

Yang X, Zhou T, Wang X, Xia Y, Cao X, Cheng X, Cao Y, Ma P, Ma H, Qin A, Zhao J
International journal of biological sciences 2023;19(15):4709-4725

Ufmylation on UFBP1 alleviates non-alcoholic fatty liver disease by modulating hepatic endoplasmic reticulum stress.

Mao Z, Ma X, Jing Y, Shen M, Ma X, Zhu J, Liu H, Zhang G, Chen F
Cell death & disease 2023 Sep 2;14(9):584

UFMylation of HRD1 regulates endoplasmic reticulum homeostasis.

Luo H, Jiao QB, Shen CB, Gong WY, Yuan JH, Liu YY, Chen Z, Liu J, Xu XL, Cong YS, Zhang XW
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Nov;37(11):e23221

DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination.

Wang X, Zhou T, Yang X, Cao X, Jin G, Zhang P, Guo J, Rong K, Li B, Hu Y, Liu K, Ma P, Qin A, Zhao J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 May;10(14):e2204438

Excessive ER-phagy contributes to ochratoxin A-induced apoptosis.

Deng H, Chen W, Zhang B, Zhang Y, Han L, Zhang Q, Yao S, Wang H, Shen XL
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Jun;176:113793

Ufmylation of UFBP1 Is Dispensable for Endoplasmic Reticulum Stress Response, Embryonic Development, and Cardiac and Intestinal Homeostasis.

Tandra V, Anderson T, Ayala JD, Weintraub NL, Singh N, Li H, Li J
Cells 2023 Jul 25;12(15)

Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control.

Ishimura R, Ito S, Mao G, Komatsu-Hirota S, Inada T, Noda NN, Komatsu M
Science advances 2023 Aug 18;9(33):eadh3635

A neuroprotective role of Ufmylation through Atg9 in the aging brain of Drosophila.

Li H, Yu Z, Niu Z, Cheng Y, Wei Z, Cai Y, Ma F, Hu L, Zhu J, Zhang W
Cellular and molecular life sciences : CMLS 2023 Apr 22;80(5):129

Deficiency of Murine UFM1-Specific E3 Ligase Causes Microcephaly and Inflammation.

Zhang J, Zhu H, Liu S, Quintero M, Zhu T, Xu R, Cai Y, Han Y, Li H
Molecular neurobiology 2022 Oct;59(10):6363-6372

A non-canonical scaffold-type E3 ligase complex mediates protein UFMylation.

Peter JJ, Magnussen HM, DaRosa PA, Millrine D, Matthews SP, Lamoliatte F, Sundaramoorthy R, Kopito RR, Kulathu Y
The EMBO journal 2022 Nov 2;41(21):e111015

The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.

Ishimura R, El-Gowily AH, Noshiro D, Komatsu-Hirota S, Ono Y, Shindo M, Hatta T, Abe M, Uemura T, Lee-Okada HC, Mohamed TM, Yokomizo T, Ueno T, Sakimura K, Natsume T, Sorimachi H, Inada T, Waguri S, Noda NN, Komatsu M
Nature communications 2022 Dec 21;13(1):7857

Ufbp1, a Key Player of Ufm1 Conjugation System, Protects Against Ketosis-Induced Liver Injury via Suppressing Smad3 Activation.

Chen F, Sheng L, Xu C, Li J, Ali I, Li H, Cai Y
Frontiers in cell and developmental biology 2021;9:676789

A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy.

Ni M, Afroze B, Xing C, Pan C, Shao Y, Cai L, Cantarel BL, Pei J, Grishin NV, Hewson S, Knight D, Mahida S, Michel D, Tarnopolsky M, Poduri A, Rotenberg A, Sondheimer N, DeBerardinis RJ
Genetics in medicine : official journal of the American College of Medical Genetics 2021 May;23(5):900-908

Ufmylation regulates granulosa cell apoptosis via ER stress but not oxidative stress during goat follicular atresia.

Zhang X, Yu T, Guo X, Zhang R, Jia Y, Shang C, Wang A, Jin Y, Lin P
Theriogenology 2021 Jul 15;169:47-55

Cdk5rap3 is essential for intestinal Paneth cell development and maintenance.

Quintero M, Liu S, Xia Y, Huang Y, Zou Y, Li G, Hu L, Singh N, Blumberg R, Cai Y, Xu H, Li H
Cell death & disease 2021 Jan 27;12(1):131

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cao Y, Li R, Shen M, Li C, Zou Y, Jiang Q, Liu S, Lu C, Li H, Liu H, Cai Y
Cell death & disease 2021 Apr 20;12(5):416

UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination.

Liu J, Guan D, Dong M, Yang J, Wei H, Liang Q, Song L, Xu L, Bai J, Liu C, Mao J, Zhang Q, Zhou J, Wu X, Wang M, Cong YS
Nature cell biology 2020 Sep;22(9):1056-1063

A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann JA, Corn JE
Cell 2020 Mar 19;180(6):1160-1177.e20

UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis.

Wang L, Xu Y, Rogers H, Saidi L, Noguchi CT, Li H, Yewdell JW, Guydosh NR, Ye Y
Cell research 2020 Jan;30(1):5-20

UFMylation is associated with LPS-induced inflammatory response in goat endometrial epithelial cells.

Xiao J, Liu S, Yu T, Zhang R, Guo X, Jia Y, Shang C, Wang A, Jin Y, Lin P
Reproduction in domestic animals = Zuchthygiene 2020 Dec;55(12):1725-1734

Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation.

Cai Y, Zhu G, Liu S, Pan Z, Quintero M, Poole CJ, Lu C, Zhu H, Islam B, Riggelen JV, Browning D, Liu K, Blumberg R, Singh N, Li H
Cell discovery 2019;5:7

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Zhu H, Bhatt B, Sivaprakasam S, Cai Y, Liu S, Kodeboyina SK, Patel N, Savage NM, Sharma A, Kaufman RJ, Li H, Singh N
Nature communications 2019 Mar 6;10(1):1084

Salubrinal, a novel inhibitor of eIF-2α dephosphorylation, promotes erythropoiesis at early stage targeted by ufmylation pathway.

Chen F, Xing C, Zhang W, Li J, Hu T, Li L, Li H, Cai Y
Journal of cellular physiology 2019 Aug;234(10):18560-18570

Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer.

Lin JX, Xie XS, Weng XF, Zheng CH, Xie JW, Wang JB, Lu J, Chen QY, Cao LL, Lin M, Tu RH, Li P, Huang CM
World journal of gastroenterology 2018 Sep 14;24(34):3898-3907

Proteomic and Biochemical Analyses Reveal a Novel Mechanism for Promoting Protein Ubiquitination and Degradation by UFBP1, a Key Component of Ufmylation.

Zhu Y, Lei Q, Li D, Zhang Y, Jiang X, Hu Z, Xu G
Journal of proteome research 2018 Apr 6;17(4):1509-1520

A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1α stability.

Liu J, Wang Y, Song L, Zeng L, Yi W, Liu T, Chen H, Wang M, Ju Z, Cong YS
Nature communications 2017 Jan 27;8:14186

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The DDRGK1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human DDRGK1. This antibody recognizes human, mouse antigen. The DDRGK1 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 21445-1-AP.pdf

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