H00001731-M03

antibody from Abnova Corporation

Targeting: SEPTIN1 DIFF6, PNUTL3, SEPT1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001731-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001731-M03, RRID:AB_1137026

Product name

SEPT1 monoclonal antibody (M03), clone 1F12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant SEPT1.

Antigen sequence

MDKEYVGFAALPNQLHRKSVKKGFDFTLMVAGESG
LGKSTLINSLFLTNLYEDRQVPEASARLTQTLAIE
RRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLP
VVKFIEEQFEQYLRDESGLNRKNIQDSRVHCCLYF
ISPFGRGLRPLDVAFLRAVHEKVNIIPVIGKADAL
MPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDE
DFKRQDAEMKESIPFAVVGSCEVVRDGGNRPVRGR
RYSWGTVEVENPHHCDFLNLRRMLVQTHLQDLKEV
THDLLYEGYRARRLQSLARPGARDRASRSKLSRQS
ATEIPLPMLPLADTEKLIREKDEELRRMQEMLEKM
QAQMQRSQAQGEQSDAL


Isotype

IgG

Antibody clone number

1F12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12.Lane 1: SEPT1 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SEPT1 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SEPT1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SEPT1 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol