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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA038314 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA038314, RRID:AB_10672587
- Product name
- Anti-CALCOCO1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human CALCOCO1, Gene description: calcium binding and coiled-coil domain 1, Alternative Gene Names: calphoglin, Cocoa, KIAA1536, Validated applications: WB, ICC, IHC, Uniprot ID: Q9P1Z2, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy
CALCOCO 1 acts with VAMP ‐associated proteins to mediate ER ‐phagy
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Nthiga T, Shrestha B, Bruun J, Larsen K, Lamark T, Johansen T
Journal of Cell Biology 2021;220(6)
Journal of Cell Biology 2021;220(6)
CALCOCO 1 acts with VAMP ‐associated proteins to mediate ER ‐phagy
Nthiga T, Kumar Shrestha B, Sjøttem E, Bruun J, Bowitz Larsen K, Bhujabal Z, Lamark T, Johansen T
The EMBO Journal 2020;39(15)
The EMBO Journal 2020;39(15)
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and CALCOCO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412204).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
- Sample type
- Human
