Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF3954 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PTP1B/PTPN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat PTP1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human PTP1B is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Intranasal Targeting of Hypothalamic PTP1B and TCPTP Reinstates Leptin and Insulin Sensitivity and Promotes Weight Loss in Obesity.
Pathologic Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell Carcinoma.
Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2.
Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia.
Dodd GT, Xirouchaki CE, Eramo M, Mitchell CA, Andrews ZB, Henry BA, Cowley MA, Tiganis T
Cell reports 2019 Sep 10;28(11):2905-2922.e5
Cell reports 2019 Sep 10;28(11):2905-2922.e5
Pathologic Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell Carcinoma.
Xu Y, Taylor P, Andrade J, Ueberheide B, Shuch B, Glazer PM, Bindra RS, Moran MF, Linehan WM, Neel BG
Cancer research 2018 Dec 1;78(23):6539-6548
Cancer research 2018 Dec 1;78(23):6539-6548
Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2.
Tsutsumi R, Harizanova J, Stockert R, Schröder K, Bastiaens PIH, Neel BG
Nature communications 2017 Sep 6;8(1):466
Nature communications 2017 Sep 6;8(1):466
Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia.
Tsunekawa T, Banno R, Mizoguchi A, Sugiyama M, Tominaga T, Onoue T, Hagiwara D, Ito Y, Iwama S, Goto M, Suga H, Sugimura Y, Arima H
EBioMedicine 2017 Feb;16:172-183
EBioMedicine 2017 Feb;16:172-183
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse/Rat PTP1B by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and recombinant mouse PTP1B 5 ng/lane) were included. A specific band for PTP1B was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse PTP1B by Simple WesternTM. Simple Western lane view shows lysates of Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 56 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.