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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [2]
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- Product number
- AF3954 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat PTP1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat PTP1B in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human PTP1B is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P35821- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Pathologic Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell Carcinoma.
Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2.
Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia.
Xu Y, Taylor P, Andrade J, Ueberheide B, Shuch B, Glazer PM, Bindra RS, Moran MF, Linehan WM, Neel BG
Cancer research 2018 Dec 1;78(23):6539-6548
Cancer research 2018 Dec 1;78(23):6539-6548
Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2.
Tsutsumi R, Harizanova J, Stockert R, Schröder K, Bastiaens PIH, Neel BG
Nature communications 2017 Sep 6;8(1):466
Nature communications 2017 Sep 6;8(1):466
Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia.
Tsunekawa T, Banno R, Mizoguchi A, Sugiyama M, Tominaga T, Onoue T, Hagiwara D, Ito Y, Iwama S, Goto M, Suga H, Sugimura Y, Arima H
EBioMedicine 2017 Feb;16:172-183
EBioMedicine 2017 Feb;16:172-183
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse/Rat PTP1B by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.3 µg/mL Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human PTP1B (Catalog # 1366-PT) and recombinant mouse PTP1B 5 ng/lane) were included. A specific band for PTP1B was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse PTP1B by Simple WesternTM. Simple Western lane view shows lysates of Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTP1B at approximately 56 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat PTP1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3954) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
