Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA503038 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503038, RRID:AB_11125050
- Product name
- PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)
- Antibody type
- Monoclonal
- Description
- PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PTPN1
- Isotype
- IgG
- Antibody clone number
- OTI1A2
- Vial size
- 100 µl
- Concentration
- 0.72 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PTPN1 mouse monoclonal antibody (TA503038) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1(RC204902).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PTPN1 antibody(TA503038),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PTPN1 antibody(TA503038),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTPN1 antibody(TA503038), and then analyzed by flow cytometry.
- Validation comment
- FC
