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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- NBP1-28691 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28691, RRID:AB_1913979
- Product name
- Rabbit Polyclonal PTP1B/PTPN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Effect of oxidative stress on protein tyrosine phosphatase 1B in scleroderma dermal fibroblasts.
Tsou PS, Talia NN, Pinney AJ, Kendzicky A, Piera-Velazquez S, Jimenez SA, Seibold JR, Phillips K, Koch AE
Arthritis and rheumatism 2012 Jun;64(6):1978-89
Arthritis and rheumatism 2012 Jun;64(6):1978-89
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: PTP1B/PTPN1 Antibody [NBP1-28691] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PTPN1 antibody NBP1-28691 used for IP at 10 ug/mg lysate. PTPN1 was also immunoprecipitated by rabbit anti-PTPN1 antibody NBP1-28692, which recognizes a downstream epitope. For blotting immunoprecipitated PTPN1, NBP1-28691 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
