Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R33483 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NEIL1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NEIL1 antibody is suitable for use in Immunohistochemistry/Western blot/Immunofluorescence/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the NEIL1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) human Daudi cell lysate without immunizing peptide, B) human Daudi cell lysate with immunizing peptide, C) human U937 cell lysate without immunizing peptide, D) human U937 cell lysate with immunizing peptide, E) human HEK293 cell lysate (nuclear fraction) without immunizing peptide, F) human HEK293 cell lysate (nuclear fraction) with immunizing peptide, with NEIL1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human U937 cells with NEIL1 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placenta with NEIL1 antibody at 5ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.