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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- AF7970 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Shrew-1/AJAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Shrew-1/AJAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human AJAP1 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Complex formation of APP with GABA(B) receptors links axonal trafficking to amyloidogenic processing.
Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile.
Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
Dinamarca MC, Raveh A, Schneider A, Fritzius T, Früh S, Rem PD, Stawarski M, Lalanne T, Turecek R, Choo M, Besseyrias V, Bildl W, Bentrop D, Staufenbiel M, Gassmann M, Fakler B, Schwenk J, Bettler B
Nature communications 2019 Mar 22;10(1):1331
Nature communications 2019 Mar 22;10(1):1331
Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile.
Klemmt PA, Resch E, Smyrek I, Engels K, Stelzer EH, Starzinski-Powitz A
Biology open 2016 Nov 15;5(11):1607-1619
Biology open 2016 Nov 15;5(11):1607-1619
Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
Schwenk J, Pérez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca MC, Hanuschkin A, Bildl W, Klingauf J, Gassmann M, Schulte U, Bettler B, Fakler B
Nature neuroscience 2016 Feb;19(2):233-42
Nature neuroscience 2016 Feb;19(2):233-42
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse Shrew-1/AJAP1 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (thalamus/hypothalamus) tissue, and mouse pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Shrew-1/AJAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7970) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shrew-1/ AJAP1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
