PA5-87782

antibody from Invitrogen Antibodies

Targeting: ICMT HSTE14, PCCMT, PPMT

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87782

Provider

Invitrogen Antibodies

Product name

ICMT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KAAMFTAGSN FNHVVQNEKS DTHTLVTSGV YAWFRHPSYV GWFYWSIGTQ VMLCNPICGV SYALTVWRFF RDRTEEEEIS LIHFFGEEYL EYKKRVPTGL PFIKGVKVDL; Positive Samples: U-87MG, K-562, HeLa, THP-1, Mouse testis, Mouse lung, Rat testis; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.34 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ICMT in extracts of various cell lines. Samples were incubated with ICMT Polyclonal antibody (Product # PA5-87782) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ICMT in paraffin-embedded rat heart. Samples were incubated with ICMT Polyclonal antibody (Product # PA5-87782) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ICMT in paraffin-embedded rat testis. Samples were incubated with ICMT Polyclonal antibody (Product # PA5-87782) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ICMT in paraffin-embedded rat heart. Samples were incubated with ICMT Polyclonal antibody (Product # PA5-87782) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ICMT in paraffin-embedded rat testis. Samples were incubated with ICMT Polyclonal antibody (Product # PA5-87782) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.