Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- BS-8019R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ICMT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BxPC-3 Cells (Human pancreas adenocarcinoma) were fixed with 4% PFA (10 min at room temperature). The cells were then incubated in 10% goat serum to block non-specific protein-protein interactions for 20 min at 37°C. Then the cells were probed with ICMT Polyclonal Antibody, Unconjugated (bs-8019R) at 1:200 for 60 minutes at room temperature followed by incubation with a Goat Anti-Rabbit IgG, Cy5 Conjugated (bs-0295G-Cy5) secondary antibody at a 1:200 dilution for 40 minutes at 37°C. DAPI (5 µg/mL; blue) was used to stain the cell nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with ICMT Polyclonal Antibody, Unconjugated (bs-8019R) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.