Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-49452 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HLA-DQA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of HLA-DQA1 in mouse embryonic fibroblast NIH/3T3 cells. Cells were fixed, permeabilized and stained with HLA-DQA1 polyclonal antibody (Product # PA5-49452) at a 1:25 dilution, followed by Dylight® 488-conjugated goat rabbit IgG (NK179883) secondary antibody at 1:200 dilution (green). The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HLA-DQA1 in K562 cells. Cells were fixed, permeabilized, incubated in 2% BSA, and stained with a HLA-DQA1 polyclonal antibody (Product # PA5-49452, red line) at a 1:25 dilution. Following incubation with the primary antibody for 1 hour at 37°C, cells were incubated with Alexa Fluor® 488 goat rabbit lgG (H+L) at 1:400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.