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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB10087 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10087, RRID:AB_1676178
- Product name
- Asap1 (phospho Y782) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Asap1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling.
The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1.
The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.
Liu Y, Yerushalmi GM, Grigera PR, Parsons JT
The Journal of biological chemistry 2005 Mar 11;280(10):8884-92
The Journal of biological chemistry 2005 Mar 11;280(10):8884-92
The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1.
Kruljac-Letunic A, Moelleken J, Kallin A, Wieland F, Blaukat A
The Journal of biological chemistry 2003 Aug 8;278(32):29560-70
The Journal of biological chemistry 2003 Aug 8;278(32):29560-70
The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.
Liu Y, Loijens JC, Martin KH, Karginov AV, Parsons JT
Molecular biology of the cell 2002 Jun;13(6):2147-56
Molecular biology of the cell 2002 Jun;13(6):2147-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent microscopy using Asap1 (phospho Y782) polyclonal antibody (Cat # PAB10087) shows detection of phosphorylated Asap1 present in mouse NIH/3T3 cells transfected with activated Src.Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Asap1 (phospho Y782) polyclonal antibody (Cat # PAB10087) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
