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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- PAB10088 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10088, RRID:AB_1671696
- Product name
- Asap1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Asap1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling.
The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1.
The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.
Liu Y, Yerushalmi GM, Grigera PR, Parsons JT
The Journal of biological chemistry 2005 Mar 11;280(10):8884-92
The Journal of biological chemistry 2005 Mar 11;280(10):8884-92
The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1.
Kruljac-Letunic A, Moelleken J, Kallin A, Wieland F, Blaukat A
The Journal of biological chemistry 2003 Aug 8;278(32):29560-70
The Journal of biological chemistry 2003 Aug 8;278(32):29560-70
The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.
Liu Y, Loijens JC, Martin KH, Karginov AV, Parsons JT
Molecular biology of the cell 2002 Jun;13(6):2147-56
Molecular biology of the cell 2002 Jun;13(6):2147-56
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Immunofluorescent microscopy using Asap1 polyclonal antibody (Cat # PAB10088) shows detection of Asap1 present in mouse NIH/3T3 cells transfected with activated Src.Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
- Validation comment
- Immunofluorescence
