Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA018215 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA018215, RRID:AB_1850443
- Product name
- Anti-GUCA2A
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human GUCA2A, Gene description: guanylate cyclase activator 2A (guanylin), Alternative Gene Names: GUCA2, STARA, Validated applications: WB, IHC, Uniprot ID: Q02747, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references A β-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer
Silencing the intestinal GUCY2C tumor suppressor axis requires APC loss of heterozygosity
APC-β-catenin-TCF signaling silences the intestinal guanylin-GUCY2C tumor suppressor axis
Silencing the GUCA2A-GUCY2C tumor suppressor axis in CIN, serrated, and MSI colorectal neoplasia
Cellular localization of guanylin and uroguanylin mRNAs in human and rat duodenal and colonic mucosa.
The guanylate cyclase-C signaling pathway is down-regulated in inflammatory bowel disease
The Paracrine Hormone for the GUCY2C Tumor Suppressor, Guanylin, Is Universally Lost in Colorectal Cancer
Rappaport J, Entezari A, Caspi A, Caksa S, Jhaveri A, Stanek T, Ertel A, Kupper J, Fortina P, McMahon S, Jaynes J, Snook A, Waldman S
Cellular and Molecular Gastroenterology and Hepatology 2022;13(4):1276-1296
Cellular and Molecular Gastroenterology and Hepatology 2022;13(4):1276-1296
Silencing the intestinal GUCY2C tumor suppressor axis requires APC loss of heterozygosity
Pattison A, Barton J, Entezari A, Zalewski A, Rappaport J, Snook A, Waldman S
Cancer Biology & Therapy 2020;21(9):799-805
Cancer Biology & Therapy 2020;21(9):799-805
APC-β-catenin-TCF signaling silences the intestinal guanylin-GUCY2C tumor suppressor axis
Blomain E, Rappaport J, Pattison A, Bashir B, Caparosa E, Stem J, Snook A, Waldman S
Cancer Biology & Therapy 2020;21(5):441-451
Cancer Biology & Therapy 2020;21(5):441-451
Silencing the GUCA2A-GUCY2C tumor suppressor axis in CIN, serrated, and MSI colorectal neoplasia
Bashir B, Merlino D, Rappaport J, Gnass E, Palazzo J, Feng Y, Fearon E, Snook A, Waldman S
Human Pathology 2019;87
Human Pathology 2019;87
Cellular localization of guanylin and uroguanylin mRNAs in human and rat duodenal and colonic mucosa.
Brenna Ø, Furnes MW, Munkvold B, Kidd M, Sandvik AK, Gustafsson BI
Cell and tissue research 2016 Aug;365(2):331-41
Cell and tissue research 2016 Aug;365(2):331-41
The guanylate cyclase-C signaling pathway is down-regulated in inflammatory bowel disease
Brenna Ø, Bruland T, Furnes M, Granlund A, Drozdov I, Emgård J, Brønstad G, Kidd M, Sandvik A, Gustafsson B
Scandinavian Journal of Gastroenterology 2015;50(10):1241-1252
Scandinavian Journal of Gastroenterology 2015;50(10):1241-1252
The Paracrine Hormone for the GUCY2C Tumor Suppressor, Guanylin, Is Universally Lost in Colorectal Cancer
Wilson C, Lin J, Li P, Snook A, Gong J, Sato T, Liu C, Girondo M, Rui H, Hyslop T, Waldman S
Cancer Epidemiology, Biomarkers & Prevention 2014;23(11):2328-2337
Cancer Epidemiology, Biomarkers & Prevention 2014;23(11):2328-2337
No comments: Submit comment
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and gUCA2A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409429).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human small intestine and liver tissues using HPA018215 antibody. Corresponding GUCA2A RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
