Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-18927 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18927, RRID:AB_1625577
- Product name
- Rabbit Polyclonal BAT3/BAG6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BAT3/BAG6 Antibody [NBP1-18927] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18927 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18928, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BAT3/BAG6 Antibody [NBP1-18927] - Detection of human BAT3 by western blot. Samples: Nuclear extract from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-BAT3 antibody NBP1-18927 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BAT3/BAG6 Antibody [NBP1-18927] - Detection of human BAT3 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAT3 antibody NBP1-18927 used for IP at 6 ug per reaction. BAT3 was also immunoprecipitated by rabbit anti-BAT3 antibody NBP1-18928. For blotting immunoprecipitated BAT3, NBP1-18927 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.