Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- ARP32760_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP32760_P050, RRID:AB_2046468
- Product name
- KLF2 antibody - middle region (ARP32760_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the middle region of human KLF2
- Description
- Affinity Purified
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Porcine, Zebrafish
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis using Anti-KLF2 antibody detects protein at predicted molecular weight using 721_B Cells as a positive control. Tissue/Lysate Concentration: 1ug/ml Antibody Concentration: 1.0ug/ml
- Sample type
- 721_B Cells
- Validation comment
- Western blot analysis detects protein at predicted molecular weight
- Primary Ab dilution
- 1.0ug/ml
- Other comments
- Lysate concentration 1ug/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Paraffin Embedded Lung Tissue using Anti-KLF2 antibody at a concentration of 4-8µg/ml
- Sample type
- Lung Tissue
- Validation comment
- The subcellular location is supported by literature.
- Primary Ab dilution
- 4-8µg/ml
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Paraffin Embedded Colon, myenteric plexus using Anti-KLF2 antibody at a concentration of 4-8µg/ml
- Sample type
- Colon, myenteric plexus
- Validation comment
- The subcellular location is supported by literature.
- Primary Ab dilution
- 4-8µg/ml
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of Paraffin Embedded Brain Tissue, cerebellum using Anti-KLF2 antibody at a concentration of 4-8µg/ml
- Sample type
- Brain Tissue, cerebellum
- Validation comment
- The subcellular location is supported by literature.
- Primary Ab dilution
- 4-8µg/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Serum response in HCT116 cells at 2 Egr1 loci over 3 times points, plus and minus blocking peptide.