NB100-65332
antibody from Novus Biologicals
Targeting: PDCD1LG2
B7-DC, bA574F11.2, Btdc, CD273, PD-L2, PDL2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- NB100-65332 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65332, RRID:AB_2162001
- Product name
- Rat Monoclonal PD-L2/B7-DC/PDCD1LG2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-65332 recognizes mouse CD273, a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Antibodies produced by clone TY25 are reported to block the binding of CD273 (B7-DC) to it's receptor.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.125 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) [NB100-65332] - Analysis using the PE conjugate of NB100-65332. Staining of non-transfected (left) and mouse B7-DC-transfected (right) cells with 0.25 ug of Rat IgG2a kappa Isotype Control PE (NBP1-43780) (open histogram) or 0.06 ug of Anti-Mouse CD273 (B7-DC) PE (filled histogram).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PD-L2/B7-DC/PDCD1LG2 Antibody (TY25) [NB100-65332] - Analysis using the Biotin conjugate of NB100-65332. Staining of B7-DC transfected cells with Anti-Mouse CD273 (B7-DC) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.