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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- UM800115 - Provider product page
- Provider
- OriGene
- Product name
- PD-L2 (PDCD1LG2) mouse monoclonal antibody, clone UMAB223
- Antibody type
- Monoclonal
- Description
- PD-L2 (PDCD1LG2) mouse monoclonal antibody, clone UMAB223
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDCD1LG2
- Isotype
- IgG
- Antibody clone number
- UMAB223
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDCD1LG2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800115 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen. Cytoplasmic and membraneous staining seen in tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PDCD1LG2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 2.5 min, UM800115)(1:500)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC224141 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDCD1LG2 antibody(UM800115), and then analyzed by flow cytometry.(1:100)
- Validation comment
- FC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1LG2 mouse monoclonal antibody (UM800115). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
- Validation comment
- 10K-CHIP
