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LearnPA5-47098
antibody from Invitrogen Antibodies
Targeting: PDCD1LG2
B7-DC, bA574F11.2, Btdc, CD273, PD-L2, PDL2
Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-47098 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD273 (B7-DC) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PD-L2 is observed and less than 1% cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b and recombinant rat B7-1 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of CD273 (B7-DC) in human lung tissue and HDLM‚2 human Hodgkins lymphoma cell line. Samples were incubated in CD273 (B7-DC) polyclonal antibody (Product # PA5-47098) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for PD‚L2/B7‚DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions.
