14-5888-82

antibody from Invitrogen Antibodies
Targeting: PDCD1LG2 B7-DC, bA574F11.2, Btdc, CD273, PD-L2, PDL2
Provider product page for 14-5888-82

Antibody data

Product number
14-5888-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD273 (B7-DC) Monoclonal Antibody (MIH18), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The MIH18 monoclonal antibody reacts with human B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. MIH18 is a useful tool for studies to elucidate the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in humans. MIH18 is a blocking antibody. Applications Reported: The MIH18 antibody has been reported for use in flow cytometric analysis. Applications Tested: The MIH18 antibody has been tested by flow cytometric analysis of mature dendritic cells and normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
MIH18
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
PDCD1LG2 protein structure - 14-5888-82 shown in red.