PA5-49513

antibody from Invitrogen Antibodies

Targeting: ARR3 ARRX

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-49513

Provider

Invitrogen Antibodies

Product name

ARR3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.38 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Mouse Models of Achromatopsia in Addressing Temporal "Point of No Return" in Gene-Therapy.

Wang NK, Liu PK, Kong Y, Levi SR, Huang WC, Hsu CW, Wang HH, Chen N, Tseng YJ, Quinn PMJ, Tai MH, Lin CS, Tsang SH
International journal of molecular sciences 2021 Jul 28;22(15)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Quantitative Real-Time RT-PCR and Western blotting of Cnga3 floxed-STOP mice. ( A ) Two sets of primer pairs were designed to span intron 2 and intron 5, respectively, in order to identify the presence of changes in expression of Cnga3 transcript in Cnga3 floxed-STOP/+ (blue) and Cnga3 floxed-STOP (red) mice. Cnga3 +/+ littermates (grey) were included as the wild-type (WT) controls. Despite an increasing trend, the level of Cnga3 amplicon upstream of the floxed - STOP cassette lacks statistical significance, whereas the level of Cnga3 amplicon downstream of the floxed - STOP cassette was significantly reduced in Cnga3 floxed-STOP/+ and Cnga3 floxed-STOP mice. The expression of target genes was normalized to mouse Rhopdosin. Results = mean +- S.E.M (n Cnga3+/+ = 5, n Cnga3 floxed-STOP/+ = 4, n Cnga3 floxed-STOP = 3; * p = 0.0294; ** p = 0.0032). ( B ) There was reduced CNGA3 protein expression in Cnga3 floxed-STOP mice compared to littermate controls, while there was no significant decrease in CNGA3 protein expression in Cnga3 cpfl5 mice. ( C ) There was no obvious change of CNGB3 protein between Cnga3 floxed-STOP and littermate controls.