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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- 39944 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2A.Z Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µg
- Concentration
- See Data Sheet
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A.Z antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (25 µg) probed with Histone H2A.Z antibody (pAb) (1 µg/mL). Specificity was verified by peptide competition. Lane 1: no peptide. Lane 2: 1 µm immunizing peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A.Z antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with Histone H2A.Z antibody at a 0.5 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A.Z antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µg of Histone H2A.Z pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A.Z antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from a human medulloblastoma cell line and 4 µL of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 18 million sequence tags were mapped to identify H2A.Z occupancy. H2A.Z is found throughout the genome, is often enriched at promoters and depleted from transcribed genes as shown in the images.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A.Z antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A.Z antibody for Histone H2A.Z. Recombinant proteins were spotted onto PVDF and probed with Histone H2A.Z antibody at a 1 µg/ml dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Recombinant H2A. Lane 2: Recombinant H2A.Z. Lane 3: Recombinant H4.
