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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- 14-9157-37 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCDH1 (Protocadherin 1) Monoclonal Antibody (CSTEM31), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This monoclonal antibody CSTEM31 reacts with human Protocadherin-1, also known as PCDH1 and cadherin-like protein 1. Applications Reported: This CSTEM31 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. (Fluorochrome-conjugated CSTEM31 is recommended for use in flow cytometery.) Applications Tested: This CSTEM31 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human iPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.5 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CSTEM31
- Vial size
- 2 mg
- Concentration
- 0.5 mg/mL
- Storage
- 4°C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2b K Isotype Control (Product # 14-4732-82) (left) or 5 µg/mL Anti-Human PCDH1 (Protocadherin 1) purified (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized human iPSCs plated on murine derived feeder cells using 5 µg/mL Mouse IgG2b K Isotype Control (Product # 14-4732-82) (left) or 5 µg/mL Anti-Human PCDH1 (Protocadherin 1) purified (right) followed by F(ab’)2-Goat anti-Mouse IgG Secondary Antibody eFluor® 660 (Product # 50-4010-82). Nuclei are stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of a mixture of normal human iPSC and the murine C2C12 cell line with Anti-Human TRA-1-60 (Podocalyxin) PerCP-eFluor® 710 (Product # 46-8863-82) and 0.25 µg Mouse IgG2b kappa Isotype Control, Purified (Product # 14-4732-82) (left) or 0.25 µg of Anti-Human PCDH1 (Protocadherin 1) Purified (right) followed by F(ab’)2-Goat anti-Mouse IgG PE (Product # 12-4010-82). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis
