PA5-29077

antibody from Invitrogen Antibodies

Targeting: CDKAL1 FLJ20342

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29077

Provider

Invitrogen Antibodies

Product name

CDKAL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Juarkat, Raji, K562, NIH-3T3. Predicted reactivity: Mouse (98%), Zebrafish (84%), Xenopus laevis (90%), Dog (96%), Cat (97%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification.

Santos MCFD, Anderson CP, Neschen S, Zumbrennen-Bullough KB, Romney SJ, Kahle-Stephan M, Rathkolb B, Gailus-Durner V, Fuchs H, Wolf E, Rozman J, de Angelis MH, Cai WM, Rajan M, Hu J, Dedon PC, Leibold EA
Nature communications 2020 Jan 15;11(1):296

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 8 Irp2 deficiency reduces the ms 2 t 6 A modification in tRNA Lys UUU causing misreading of lysine codons in proinsulin. a Secondary structure and sequence of tRNA Lys UUU with anticodon loop modifications 2-methylthio-N 6 -threonylcarbamoyl adenosine (ms 2 t 6 A37), methoxycarbonylmethyl-2-thiouridine (mcm 5 s 2 U34) and pseudouridine-39 (psi-39). The ms 2 group on ms 2 t 6 A37 is indicated by a dotted circle. Adapted from Vendeix, et al. 68 . b Western blot analysis of Cdkal1 in control, sgIrp2.1, and sgIrp2.2 INS-1 cells grown in medium with or without DFO or FAC for 18 h. beta-Actin is a loading control. Asterisk, nonspecific band. c Western blot analysis of Irp2, Cdkal1, Pcsk1, and CgA in islets isolated from 6-month old WT and Irp2 -/- mice. Gapdh is a loading control for Irp2, Cdkal1 and Pcsk1 and beta-actin is a loading control for CgA ( n = 3 mice per genotype). d - e LC-MS analysis of ms 2 t 6 A ( d ) and t 6 A ( e ) modifications in control, sgIrp2.1, and sgIrp2.2 INS-1 cells grown in medium with or without supplemental FAC for 18 h ( n >= 3 independent biological experiments). f Relative incorporation of 14 C-lysine versus 3 H-leucine in proinsulin immunoprecipitated from control, sgIrp2.1, and sgIrp2.2 INS-1 cells grown in medium with or without supplemental FAC ( n = 3 independent biological experiments). Data in d , e are expressed as means +- s.e.m., unpaired two-tailed Student's t test, * p < 0.05, ** p < 0.01 relative to control INS-1 cells; data in f a