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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Immunocytochemistry [1]
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- Product number
- PA5-62010 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLEKHG5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KGDRKSTGLK LSKKKARRRH TDDPSKECFT LKFDLNVDIE TEIVPAMKKK SLGEVLLPVF ERKGIALGKV DIYLDQSNTP LSL Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification and characterization of a new isoform of small GTPase RhoE.
Dai Y, Luo W, Yue X, Ma W, Wang J, Chang J
Communications biology 2020 Oct 15;3(1):572
Communications biology 2020 Oct 15;3(1):572
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PLEKHG5 in human cell line U-2 OS using a PLEKHG5 Polyclonal Antibody (Product # PA5-62010) shows localization to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Functional similarity between RhoE and RhoEalpha. a Co-IP assay detected binding of ROCK1 to both RhoE and RhoEalpha in HEK293 cells. b Confocal images of NIH-3T3 cells. Cells were transfected with the indicated plasmids and fixed 24 h after transfection. Flag-tagged proteins were stained with anti-flag antibody (red). Cytoskeleton actins were stained by phalloidin (green). Cell nuclei was stained by DAPI (blue). Scale bar: 10 um. c Co-IP assay detected binding of both RhoE and RhoEalpha to Syx and p190RhoGAP. HEK293 cells were transfected with indicated plasmids. Twenty four hours after transfection, cell lysates were used for Co-IP assay with anti-flag antibody.
