13606-1-AP

antibody from Proteintech Group

Targeting: FBXO22 FBX22, FISTC1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

13606-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13606-1-AP, RRID:AB_2104403

Product name

FBXO22 antibody

Antibody type

Polyclonal

Description

KD/KO validated FBXO22 antibody (Cat. #13606-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

BPC157 drives angiogenesis through FBXO22-dependent stabilization of BACH1.

Zhang J, Liu M, Ou H, Wang Z, He L, Xiao Y, Xie F, Yu D, Cao H, He W, Wang S, Zhang W, Zhang K, Zhang Y, Li M, Hao Q
Cell communication and signaling : CCS 2026 Jan 29;24(1)

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FBXO22 promotes hepatocellular carcinoma progression via paracrine myo‑inositol‑induced M2‑type polarization of macrophages.

Bai L, Xiong J, Chen S, Hu J, Zhang M, Li B, Hu J, He M
International journal of molecular medicine 2026 Feb;57(2)

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FBXO22-mediated ubiquitination of KLF10 promoting pancreatic cancer proliferation and invasion.

Liu X, He X, Hu Y, Xue Y, Jiang F, Wang X
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2026 Feb;26(1):103-113

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FBXO22 targets ubiquitination and degradation of c-Cbl in leukemia.

Li J, Ma L, Wang J, Xu W, Cheng Y, Zhang J, Cao H, Wang Y, Wang Z, Xu H, Li Y, Zhang Y
Scientific reports 2026 Feb 26;16(1)

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CSN5i-3 is an orthosteric molecular glue inhibitor of COP9 signalosome.

Shi H, Wang X, Yu C, Mao H, Jiao F, Braitbard M, Shor B, Zhang Z, Hinds TR, Cao S, Fan E, Schneidman-Duhovny D, Huang L, Zheng N
Nature 2026 Apr;652(8112):1375-1383

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Identification of a BACH1 lung cancer signature: A novel tool for understanding BACH1 biology and identifying new inhibitors.

Klenja-Skudrinja D, Ali KX, Walker D, Higgins M, Patel AA, Raj D, Creelman A, McDowall C, Taylor C, Wenta T, Larsson E, Wiel C, Sayin VI, de la Vega L
Redox biology 2025 Sep;85:103789

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mTORC1 senses glutamine and other amino acids through GCN2.

Figlia G, Müller S, Garcia-Cortizo F, Neff M, Klinke G, Poschet G, Teleman AA
The EMBO journal 2025 Sep;44(17):4825-4866

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Development of Degraders and 2-pyridinecarboxyaldehyde (2-PCA) as a recruitment Ligand for FBXO22.

Qiu T, Zhuang Z, Byun WS, Kozicka Z, Baek K, Zhong J, Thornhill AM, Ryan JK, Donovan KA, Fischer ES, Ebert BL, Gray NS
bioRxiv : the preprint server for biology 2025 Aug 20;

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E3 ubiquitin ligase FBXO22 inhibits SARS-CoV-2 replication via promoting proteasome-dependent degradation of NSP5.

Zhou Y, Feng W, Yang C, Wei X, Fan L, Wu Y, Gao X, Shen X, Zhang Z, Zhao J
Journal of medical virology 2024 Sep;96(9):e29891

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FBXO22 promotes glioblastoma malignant progression by mediating VHL ubiquitination and degradation.

Shen Z, Dong T, Yong H, Deng C, Chen C, Chen X, Chen M, Chu S, Zheng J, Li Z, Bai J
Cell death discovery 2024 Mar 23;10(1):151

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FBXO22 is a potential therapeutic target for recurrent chondrosarcoma.

Xin B, Chen H, Zhu Z, Guan Q, Bai G, Yang C, Zou W, Gao X, Li L, Liu T
Journal of bone oncology 2024 Jun;46:100605

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Distinct Perception Mechanisms of BACH1 Quaternary Structure Degrons by Two F-box Proteins under Oxidative Stress.

Cao S, Shi H, Garcia SF, Kito Y, Shi H, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, Pagano M, Zheng N
bioRxiv : the preprint server for biology 2024 Jun 3;

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Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis.

Chen Y, Zhou Y, Feng X, Wu Z, Yang Y, Rao X, Zhou R, Meng R, Dong X, Xu S, Zhang S, Wu G, Jie X
Cell death & disease 2024 Jan 31;15(1):104

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Recruitment of FBXO22 for targeted degradation of NSD2.

Nie DY, Tabor JR, Li J, Kutera M, St-Germain J, Hanley RP, Wolf E, Paulakonis E, Kenney TMG, Duan S, Shrestha S, Owens DDG, Maitland MER, Pon A, Szewczyk M, Lamberto AJ, Menes M, Li F, Penn LZ, Barsyte-Lovejoy D, Brown NG, Barsotti AM, Stamford AW, Collins JL, Wilson DJ, Raught B, Licht JD, James LI, Arrowsmith CH
Nature chemical biology 2024 Dec;20(12):1597-1607

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Dual BACH1 regulation by complementary SCF-type E3 ligases.

Goretzki B, Khoshouei M, Schröder M, Penner P, Egger L, Stephan C, Argoti D, Dierlamm N, Rada JM, Kapps S, Müller CS, Thiel Z, Mutlu M, Tschopp C, Furkert D, Freuler F, Haenni S, Tenaillon L, Knapp B, Hinniger A, Hoppe P, Schmidt E, Gutmann S, Iurlaro M, Ryzhakov G, Fernández C
Cell 2024 Dec 26;187(26):7585-7602.e25

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Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress.

Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, Guttman M, Pagano M, Zheng N
Cell 2024 Dec 26;187(26):7568-7584.e22

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TANK Binding Kinase 1 Promotes BACH1 Degradation through Both Phosphorylation-Dependent and -Independent Mechanisms without Relying on Heme and FBXO22.

Liu L, Matsumoto M, Watanabe-Matsui M, Nakagawa T, Nagasawa Y, Pang J, Callens BKK, Muto A, Ochiai K, Takekawa H, Alam M, Nishizawa H, Shirouzu M, Shima H, Nakayama K, Igarashi K
International journal of molecular sciences 2024 Apr 9;25(8)

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FBXO22 inhibits proliferation and metastasis of cervical cancer cells by mediating ubiquitination-dependent degradation of GAK.

Li S, Shi L, Wang Y, Zhang L, Chu S, Li M, Bai J, Zhu W
Experimental cell research 2023 Sep 1;430(1):113719

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Recruitment of FBXO22 for Targeted Degradation of NSD2.

Nie DY, Tabor JR, Li J, Kutera M, St-Germain J, Hanley RP, Wolf E, Paulakonis E, Kenney TMG, Duan S, Shrestha S, Owens DDG, Pon A, Szewczyk M, Lamberto AJ, Menes M, Li F, Barsyte-Lovejoy D, Brown NG, Barsotti AM, Stamford AW, Collins JL, Wilson DJ, Raught B, Licht JD, James LI, Arrowsmith CH
bioRxiv : the preprint server for biology 2023 Nov 30;

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High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer.

Wang H, Chen S, Kang W, Ding B, Cui S, Zhou L, Zhang N, Luo H, Wang M, Zhang F, Zhao Z, Guo Z, Wang C, Li L, Wang Z, Chen X, Wang Y
Discover oncology 2023 Feb 23;14(1):25

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The tRNA-GCN2-FBXO22-axis-mediated mTOR ubiquitination senses amino acid insufficiency.

Ge MK, Zhang C, Zhang N, He P, Cai HY, Li S, Wu S, Chu XL, Zhang YX, Ma HM, Xia L, Yang S, Yu JX, Yao SY, Zhou XL, Su B, Chen GQ, Shen SM
Cell metabolism 2023 Dec 5;35(12):2216-2230.e8

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Fbxo22 inhibits metastasis in triple-negative breast cancer through ubiquitin modification of KDM5A and regulation of H3K4me3 demethylation.

Li S, He J, Liao X, He Y, Chen R, Chen J, Hu S, Sun J
Cell biology and toxicology 2023 Aug;39(4):1641-1655

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Fbxo22 promotes cervical cancer progression via targeting p57(Kip2) for ubiquitination and degradation.

Lin M, Zhang J, Bouamar H, Wang Z, Sun LZ, Zhu X
Cell death & disease 2022 Sep 20;13(9):805

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Biotinylation of an acetylenic tricyclic bis(cyanoenone) lowers its potency as an NRF2 activator while creating a novel activity against BACH1.

Moreno R, Casares L, Higgins M, Ali KX, Honda T, Wiel C, Sayin VI, Dinkova-Kostova AT, de la Vega L
Free radical biology & medicine 2022 Oct;191:203-211

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BACH1 Expression Is Promoted by Tank Binding Kinase 1 (TBK1) in Pancreatic Cancer Cells to Increase Iron and Reduce the Expression of E-Cadherin.

Liu L, Matsumoto M, Matsui-Watanabe M, Ochiai K, Callens BKK, Nguyen LC, Kozuki Y, Tanaka M, Nishizawa H, Igarashi K
Antioxidants (Basel, Switzerland) 2022 Jul 27;11(8)

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A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.

Hussain M, Lu Y, Tariq M, Jiang H, Shu Y, Luo S, Zhu Q, Zhang J, Liu J
iScience 2022 Jul 15;25(7):104591

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Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.

Liu P, Cong X, Liao S, Jia X, Wang X, Dai W, Zhai L, Zhao L, Ji J, Ni D, Liu Z, Chen Y, Pan L, Liu W, Zhang J, Huang M, Liu B, Tan M
Cell death and differentiation 2022 Jan;29(1):1-13

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FBXO22 Promotes Growth and Metastasis and Inhibits Autophagy in Epithelial Ovarian Cancers via the MAPK/ERK Pathway.

Li M, Zhao X, Yong H, Shang B, Lou W, Wang Y, Bai J
Frontiers in pharmacology 2021;12:778698

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Pan-Cancer Analyses Reveal Oncogenic Role and Prognostic Value of F-Box Only Protein 22.

Chen S, Ma S, Yan J, Wang H, Ding B, Guo Z, Ma Y, Chen X, Wang Y
Frontiers in oncology 2021;11:790912

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FBXO22 Suppresses Metastasis in Human Renal Cell Carcinoma via Inhibiting MMP-9-Mediated Migration and Invasion and VEGF-Mediated Angiogenesis.

Guo F, Liu J, Han X, Zhang X, Lin T, Wang Y, Bai J, Han J
International journal of biological sciences 2019;15(3):647-656

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FBXO22 mediates polyubiquitination and inactivation of LKB1 to promote lung cancer cell growth.

Zhu XN, He P, Zhang L, Yang S, Zhang HL, Zhu D, Liu MD, Yu Y
Cell death & disease 2019 Jun 19;10(7):486

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Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.

Lignitto L, LeBoeuf SE, Homer H, Jiang S, Askenazi M, Karakousi TR, Pass HI, Bhutkar AJ, Tsirigos A, Ueberheide B, Sayin VI, Papagiannakopoulos T, Pagano M
Cell 2019 Jul 11;178(2):316-329.e18

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FBXO22 promotes the development of hepatocellular carcinoma by regulating the ubiquitination and degradation of p21.

Zhang L, Chen J, Ning D, Liu Q, Wang C, Zhang Z, Chu L, Yu C, Liang HF, Zhang B, Chen X
Journal of experimental & clinical cancer research : CR 2019 Feb 26;38(1):101

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Phosphorylation of BACH1 switches its function from transcription factor to mitotic chromosome regulator and promotes its interaction with HMMR.

Li J, Shima H, Nishizawa H, Ikeda M, Brydun A, Matsumoto M, Kato H, Saiki Y, Liu L, Watanabe-Matsui M, Iemura K, Tanaka K, Shiraki T, Igarashi K
The Biochemical journal 2018 Mar 15;475(5):981-1002

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F-Box Protein FBXO22 Mediates Polyubiquitination and Degradation of CD147 to Reverse Cisplatin Resistance of Tumor Cells.

Wu B, Liu ZY, Cui J, Yang XM, Jing L, Zhou Y, Chen ZN, Jiang JL
International journal of molecular sciences 2017 Jan 20;18(1)

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Targeted inhibition of the COP9 signalosome for treatment of cancer.

Schlierf A, Altmann E, Quancard J, Jefferson AB, Assenberg R, Renatus M, Jones M, Hassiepen U, Schaefer M, Kiffe M, Weiss A, Wiesmann C, Sedrani R, Eder J, Martoglio B
Nature communications 2016 Oct 24;7:13166

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Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ.

Hwang CY, Kim K, Choi JY, Bahn YJ, Lee SM, Kim YK, Lee C, Kwon KS
Proteomics 2014 Jan;14(1):121-32

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13606-1-AP(FBXO22 antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human tonsil using 13606-1-AP(FBXO22 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The FBXO22 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human FBXO22. This antibody recognizes human,mouse,rat antigen. The FBXO22 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

13606-1-AP.pdf

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