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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 20180-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20180-1-AP, RRID:AB_10665948
- Product name
- PSAT1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PSAT1 antibody (Cat. #20180-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin.
The breast cancer oncogene IKKε coordinates mitochondrial function and serine metabolism.
Macrophages induce malignant traits in mammary epithelium via IKKε/TBK1 kinases and the serine biosynthesis pathway.
Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes.
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Byles V, Cormerais Y, Kalafut K, Barrera V, Hughes Hallett JE, Sui SH, Asara JM, Adams CM, Hoxhaj G, Ben-Sahra I, Manning BD
Molecular metabolism 2021 Nov;53:101309
Molecular metabolism 2021 Nov;53:101309
The breast cancer oncogene IKKε coordinates mitochondrial function and serine metabolism.
Xu R, Jones W, Wilcz-Villega E, Costa AS, Rajeeve V, Bentham RB, Bryson K, Nagano A, Yaman B, Olendo Barasa S, Wang Y, Chelala C, Cutillas P, Szabadkai G, Frezza C, Bianchi K
EMBO reports 2020 Sep 3;21(9):e48260
EMBO reports 2020 Sep 3;21(9):e48260
Macrophages induce malignant traits in mammary epithelium via IKKε/TBK1 kinases and the serine biosynthesis pathway.
Wilcz-Villega E, Carter E, Ironside A, Xu R, Mataloni I, Holdsworth J, Jones W, Moreno Béjar R, Uhlik L, Bentham RB, Godinho SA, Dalli J, Grose R, Szabadkai G, Jones L, Hodivala-Dilke K, Bianchi K
EMBO molecular medicine 2020 Feb 7;12(2):e10491
EMBO molecular medicine 2020 Feb 7;12(2):e10491
Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes.
Sellers K, Allen TD, Bousamra M 2nd, Tan J, Méndez-Lucas A, Lin W, Bah N, Chernyavskaya Y, MacRae JI, Higashi RM, Lane AN, Fan TW, Yuneva MO
British journal of cancer 2019 Jul;121(1):51-64
British journal of cancer 2019 Jul;121(1):51-64
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Ben-Sahra I, Hoxhaj G, Ricoult SJH, Asara JM, Manning BD
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 20180-1-AP(PSAT1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PSAT1 antibody 20180-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 20180-1-AP(PSAT1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 20180-1-AP(PSAT1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
