Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-32920 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32920, RRID:AB_2172600
- Product name
- Rabbit Polyclonal PSAT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Regorafenib induces lethal autophagy arrest by stabilizing PSAT1 in glioblastoma.
Transcriptional profiling and biomarker identification reveal tissue specific effects of expanded ataxin-3 in a spinocerebellar ataxia type 3 mouse model.
Modulating the therapeutic response of tumours to dietary serine and glycine starvation.
PHGDH Expression Is Required for Mitochondrial Redox Homeostasis, Breast Cancer Stem Cell Maintenance, and Lung Metastasis.
Jiang J, Zhang L, Chen H, Lei Y, Zhang T, Wang Y, Jin P, Lan J, Zhou L, Huang Z, Li B, Liu Y, Gao W, Xie K, Zhou L, Nice EC, Peng Y, Cao Y, Wei Y, Wang K, Huang C
Autophagy 2020 Jan;16(1):106-122
Autophagy 2020 Jan;16(1):106-122
Transcriptional profiling and biomarker identification reveal tissue specific effects of expanded ataxin-3 in a spinocerebellar ataxia type 3 mouse model.
Toonen LJA, Overzier M, Evers MM, Leon LG, van der Zeeuw SAJ, Mei H, Kielbasa SM, Goeman JJ, Hettne KM, Magnusson OT, Poirel M, Seyer A, 't Hoen PAC, van Roon-Mom WMC
Molecular neurodegeneration 2018 Jun 22;13(1):31
Molecular neurodegeneration 2018 Jun 22;13(1):31
Modulating the therapeutic response of tumours to dietary serine and glycine starvation.
Maddocks ODK, Athineos D, Cheung EC, Lee P, Zhang T, van den Broek NJF, Mackay GM, Labuschagne CF, Gay D, Kruiswijk F, Blagih J, Vincent DF, Campbell KJ, Ceteci F, Sansom OJ, Blyth K, Vousden KH
Nature 2017 Apr 19;544(7650):372-376
Nature 2017 Apr 19;544(7650):372-376
PHGDH Expression Is Required for Mitochondrial Redox Homeostasis, Breast Cancer Stem Cell Maintenance, and Lung Metastasis.
Samanta D, Park Y, Andrabi SA, Shelton LM, Gilkes DM, Semenza GL
Cancer research 2016 Aug 1;76(15):4430-42
Cancer research 2016 Aug 1;76(15):4430-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSAT1 Antibody [NBP1-32920] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSAT1 Antibody [NBP1-32920] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSAT1 Antibody [NBP1-32920] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3] diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PSAT1 Antibody [NBP1-32920] - NCI-N87 xenograft, using PSAT1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.