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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8212 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phosphoserine aminotransferase Antibody / PSAT1
- Antibody type
- Polyclonal
- Description
- This highly specific Phosphoserine aminotransferase antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Phosphoserine aminotransferase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human 293T, 3) human HepG2, 4) human Jurkat, 5) rat brain and 6) mouse brain tissue lysate with Phosphoserine aminotransferase antibody. Predicted molecular weight: 35-40 kDa (two isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human PC-3 cells with Phosphoserine aminotransferase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human JK cells with Phosphoserine aminotransferase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Phosphoserine aminotransferase antibody.
