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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB600-263 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-263, RRID:AB_2064137
- Product name
- Rabbit Polyclonal BCL11B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB600-263 is specific for human Bcl-11b protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody NB600-263 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-263. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-262 and NB600-264.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
