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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-30192 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLE4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, Jurkat, HeLa. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.95 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TLE4 Polyclonal Antibody (Product # PA5-30192). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 7.5% SDS PAGE. TLE4 Polyclonal Antibody (Product # PA5-30192) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TLE4 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TLE4 Polyclonal Antibody (Product # PA5-30192) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
