PA5-78698

antibody from Invitrogen Antibodies

Targeting: ABCE1 OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78698

Provider

Invitrogen Antibodies

Product name

ABCE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Human Bcl-XL shares 97.9% amino acid (aa) sequence identity with both mouse and rat Bcl-XL. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human HepG2 whole cell lysates, human A431 whole cell lysates, human U2OS whole cell lysates, human Jurkat whole cell lysates, rat brain tissue lysates, mouse brian tissue lysates, mouse 4T1 whole cell lysates, mouse NIH/3T3 whole cell lysates. ICC/IF: Hela cell IP: A431 cell. Flow: U251 cell.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of ABCE1 in Hela cells using ABCE1 Polyclonal Antibody (Product # PA5-78698). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of ABCE1 in U251 cells using ABCE1 Polyclonal Antibody (Product # PA5-78698), shown in overlay histogram (blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum, and incubated with the primary antibody (1 μg/1x10^6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.