Explore
Validate
LearnPA5-30993
antibody from Invitrogen Antibodies
Targeting: ABCE1
OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30993 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ABCE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, NCI-H929, K562. Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (85%), Xenopus laevis (94%), Chicken (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.57 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ABCE1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ABCE1 Polyclonal Antibody (Product # PA5-30993) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ABCE1 Polyclonal Antibody detects ABCE1 protein by Western blot analysis. A. 30 µg K562 whole cell lysate/extract.7.5 % SDS-PAGE. ABCE1 Polyclonal Antibody (Product # PA5-30993) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ABCE1 Polyclonal Antibody detects ABCE1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: ABCE1 protein stained by ABCE1 Polyclonal Antibody (Product # PA5-30993) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ABCE1 (Product # PA5-30993) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
