PA5-30993

antibody from Invitrogen Antibodies

Targeting: ABCE1 OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30993

Provider

Invitrogen Antibodies

Product name

ABCE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, Raji, NCI-H929, K562.

Concentration

0.57 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ABCE1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ABCE1 Polyclonal Antibody (Product # PA5-30993) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ABCE1 Polyclonal Antibody detects ABCE1 protein by Western blot analysis. A. 30 µg K562 whole cell lysate/extract.7.5 % SDS-PAGE. ABCE1 Polyclonal Antibody (Product # PA5-30993) dilution: 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ABCE1 Polyclonal Antibody detects ABCE1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: ABCE1 protein stained by ABCE1 Polyclonal Antibody (Product # PA5-30993) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ABCE1 (Product # PA5-30993) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.