500-P212-100UG

antibody from Invitrogen Antibodies

Targeting: CCN5 CT58, CTGF-L, WISP-2, WISP2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P212-100UG

Provider

Invitrogen Antibodies

Product name

WISP2 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTGFL/WISP-2. Anti-Human CTGFL/WISP-2-specific antibody was purified by affinity chromatography employing an immobilized Human CTGFL/WISP-2 matrix. Sandwich ELISA: To detect Human CTGFL/WISP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CTGFL/WISP-2 (500-P212Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGFL/WISP-2. Western Blot: To detect Human CTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Rabbit

Vial size

100 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human CTGFL/WISP-2 by Western Blot analysis WISP2 Polyclonal Antibody (Product # 500-P212-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human CTGFL/WISP-2 by Western Blot analysis WISP2 Polyclonal Antibody (Product # 500-P212-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human CTGFL/WISP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of WISP2 Polyclonal Antibody (Product # 500-P212-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech WISP2 Polyclonal Antibody, Biotin (Product # 500-P212BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGFL/WISP-2.