H00055937-M01

antibody from Abnova Corporation

Targeting: APOM G3a, NG20

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00055937-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00055937-M01, RRID:AB_426027

Product name

APOM monoclonal antibody (M01), clone 1F10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant APOM.

Antigen sequence

CPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTK
EELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKD
GLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSS
CPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFK
SLTSCLDSKAFLLTPRNQEACELSNN


Isotype

IgG

Antibody clone number

1F10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Effects of simvastatin on apolipoprotein M in vivo and in vitro.

Zhang X, Mao S, Luo G, Wei J, Berggren-Söderlund M, Nilsson-Ehle P, Xu N
Lipids in health and disease 2011 Jul 5;10:112

---

Liver X receptor agonist downregulates hepatic apoM expression in vivo and in vitro.

Zhang X, Zhu Z, Luo G, Zheng L, Nilsson-Ehle P, Xu N
Biochemical and biophysical research communications 2008 Jun 20;371(1):114-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10.Lane 1: APOM transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged APOM is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol