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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [2]
- ELISA [1]
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- Product number
- H00055937-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055937-M01, RRID:AB_536861
- Product name
- Mouse Monoclonal Apolipoprotein M/ApoM Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. APOM - apolipoprotein M
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references 17β-estradiol regulates the expression of apolipoprotein M through estrogen receptor α-specific binding motif in its promoter.
Effects of simvastatin on apolipoprotein M in vivo and in vitro.
Liver X receptor agonist downregulates hepatic apoM expression in vivo and in vitro.
Wei J, Yu Y, Luo GH, Feng YH, Shi YP, Zhang J, Mu QF, Yu MM, Pan LL, Berggren-Söderlund M, Nilsson-Ehle P, Zhang XY, Xu N
Lipids in health and disease 2017 Mar 31;16(1):66
Lipids in health and disease 2017 Mar 31;16(1):66
Effects of simvastatin on apolipoprotein M in vivo and in vitro.
Zhang X, Mao S, Luo G, Wei J, Berggren-Söderlund M, Nilsson-Ehle P, Xu N
Lipids in health and disease 2011 Jul 5;10:112
Lipids in health and disease 2011 Jul 5;10:112
Liver X receptor agonist downregulates hepatic apoM expression in vivo and in vitro.
Zhang X, Zhu Z, Luo G, Zheng L, Nilsson-Ehle P, Xu N
Biochemical and biophysical research communications 2008 Jun 20;371(1):114-7
Biochemical and biophysical research communications 2008 Jun 20;371(1):114-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM over-expressed 293 cell line, cotransfected with APOM Validated Chimera RNAi ( Cat # H00055937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with APOM monoclonal antibody (M01), clone 1F10 (Cat # H00055937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10.Lane 1: APOM transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Apolipoprotein M/ApoM Antibody (1F10) [H00055937-M01] - Detection limit for recombinant GST tagged APOM is approximately 0.3ng/ml as a capture antibody.
