A304-605A-T

antibody from Invitrogen Antibodies

Targeting: OTUD4 DUBA6, HSHIN1, KIAA1046

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A304-605A-T

Provider

Invitrogen Antibodies

Product name

OTUD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse OTUD4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-OTUD4 antibody (Product # A304-605A; lot A304-605A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human OTUD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-OTUD4 antibody (Product # A304-605A; lot A304-605A-1) used for IP at 6 µg per reaction. OTUD4 was also immunoprecipitated by rabbit anti-OTUD4 antibody (Product # A304-606A). For blotting immunoprecipitated OTUD4, Product # A304-605A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.