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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30680 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIN3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116.
- Concentration
- 0.96 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SIN3B Polyclonal Antibody (Product # PA5-30680). Sample (30 µg of whole cell lysate). Lane A: HCT116. 5% SDS PAGE. SIN3B Polyclonal Antibody (Product # PA5-30680) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SIN3B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B Polyclonal Antibody (Product # PA5-30680) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using SIN3B (Product # PA5-30680) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
