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Western blot
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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C673718 - Provider product page
- Provider
- LSBio
- Product name
- SNX12 Antibody LS-C673718
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: SNX12 antibody at 2.83µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 19 kDa Observed band size: 20 kDa
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: SNX12 antibody at 2.83µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20, 19 kDa Observed band size: 20 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating SNX12 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of SNX12 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: SNX12 Antibody (6µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating SNX12 in A549 whole cell lysate Lane 1: Rabbit control IgG instead of SNX12 Antibody in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: SNX12 Antibody (6µg) + A549 whole cell lysate (500µg) Lane 3: A549 whole cell lysate (20µg)
