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Flow cytometry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- MA5-24286 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL2RG Monoclonal Antibody (633162)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with recombinant human IL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross-reactivity with rhIL-2 R alpha is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 633162
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of recombinant Human IL-2 stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Common γ Chain/IL-2 Rγ Monoclonal Antibody (Product # MA5-24286). The ND50 is typically 0.08-0.4 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IL2RG in Human peripheral blood lymphocytes. Samples were incubated in IL2RG monoclonal antibody (Product # MA5-24286) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL2RG in MO7e human megakaryocytic leukemic cell line. Samples were incubated in IL2RG monoclonal antibody (Product # MA5-24286). Recombinant Human IL‚2 stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‚2 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Common γ Chain/IL‚2 Rγ Monoclonal Antibody. The ND50 is typically 0.08-0.4 µg/mL.
