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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
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- Product number
- PA5-119907 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL2RG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse spleen tissue lysates, SH-SY5Y, SiHa, human spleen tissue, human tonsil tissue. Subcellular Location: Cell membrane, Cell surface.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SH-SY5Y cells using IL2RG Polyclonal Antibody (Product # PA5-119907) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with IL2RG Polyclonal Antibody at 1:200 dilution in 2% BSA overnight at 4 8451. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SiHa cells using IL2RG Polyclonal Antibody (Product # PA5-119907) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with IL2RG Polyclonal Antibody at 1:200 dilution in 2% BSA overnight at 4°C. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
