MA5-24844

antibody from Invitrogen Antibodies

Targeting: TSPO BZRP, DBI, IBP, MBR, mDRC, PBR, pk18, PKBS

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: MA5-24844 - Provider product page
Provider: Invitrogen Antibodies
Product name: TSPO Recombinant Rabbit Monoclonal Antibody (4H2)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: 4H2
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TSPO protein structure - MA5-24844 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of TSPO in SW480 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS and incubated with TSPO monoclonal antibody (Product # MA5-24844).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of TSPO in PC-3M cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with TSPO monoclonal antibody (Product # MA5-24844).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TSPO in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with TSPO monoclonal antibody (Product # MA5-24844).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TSPO was performed using THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TSPO Recombinant Rabbit Monoclonal Antibody (Product # MA5-24844) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: green) in THP1 cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells showing nuclear membrane and cytosol localization for TSPO. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 3 TSPO-Iba-1 double label immunoreactivity is abundant in cortical and hippocampal regions. a - f Representative samples of double-label immunostained 5 mum brain sections for TSPO (red) and Iba-1 (brown). a Multiple layers (i-vi) of the cortex can be seen from buffer-OPN + group. b Midbrain region from HIV-OPN + group, showing a diversity of microglia morphologies. c HE-stained hippocampal brain regions (i) Buffer-OPN + , (ii) Buffer-OPN - , (iii) HIV OPN + , (iv) HIV OPN - . Yellow bars, highlight the dentate gyrus and red circles indicate hippocampal fissures. d , e Hippocampus of HIV-OPN - . f Hippocampus in buffer-OPN - . g Meningeal inflammation in HIV OPN +

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 4 Robust TSPO immunoreactivity in a subset of neurons in the cerebellum. a-e Representative samples of double-label immunostained 5 mum brain sections for TSPO (red) and Iba-1 (brown) and their corresponding hematoxylin/eosin near adjacent section are shown a Buffer-OPN + , b HIV-OPN + , c HIV-OPN + , d Buffer-OPN - , and e HIV-OPN - . An ordered array of Purkinje neurons was not visible in HIV-infected mice lacking osteopontin ( e , HIV-OPN - ) compared to d HIV-OPN + . f Enlarged area from d , e is shown with the white matter, Purkinje, granular, and molecular layers of the cerebellum indicated by the arrows

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 Strong TSPO immunoreactivity in a subset of tyrosine hydroxylase+ neurons in the striatum. a-f Representative samples of double-label immunostained 5 mum brain sections for TSPO (red) and Iba-1 (brown). a Buffer OPN+, b HIV OPN+, c HIV OPN+, d Buffer OPN-, e HIV OPN-, f Buffer OPN-. g hematoxylin/eosin stained section, and serial adjacent sections stained with h TSPO and i tyrosine hydroxylase. The black ovals demarcate the region of interest in each section

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1. Quantification of TSPO protein in autopsied human brain. (a) Immunoblots of TSPO in the pooled tissue standards, in the commercial N-His-tagged recombinant human TSPO-3462H and in human adrenal samples with monoclonal (EPR5384, 1:100K and MA5-24844, 1:30K dilution) and polyclonal (PA5-75544, 1:10K) antibodies. (b) Standard curves for the recombinant TSPO. Note more abundant TSPO in adrenal than in brain for both EPR5384 and MA5-24844, polymer protein bands of the recombinant TSPO, high molecular weight non-specific reactions for PA5-75544, and detection of a TSPO fragment but not 18 kDa TSPO by PA5-75544 in human adrenal samples.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. Representative immunoblots of the regional distribution of TSPO and GFAP in autopsied human brain. A23: cingulate gyrus posterior; A24: cingulate gyrus anterior; A25: paraolfactory/subgenual gyrus; CCc: corpus callosum caudal; CCr: corpus callosum rostral; cereb: cerebellar cortex; CN: caudate; CNA: hippocampal Ammon's horn; CSTH: subthalamic nucleus; GD: dentate gyrus; GH: hippocampal gyrus; GPe: globus pallidus external; GPi: globus pallidus internal; GUNC: gyrus of uncus; hypothal: hypothalamus; ICr: internal capsule rostral; MDTH: mediodorsal thalamus; NAM: amygdala; NAV: anterior ventral nucleus of thalamus; N. basalis: nucleus basalis; NL: nucleus lateralis of thalamus; NLV: lateral ventral nucleus of thalamus; NPM: medial pulvinar of thalamus; PUT: putamen; RN: red nucleus; SBI: substantia innominata; SNpc: substantia nigra pars compacta.