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LearnPA5-18565
antibody from Invitrogen Antibodies
Targeting: TSPO
BZRP, DBI, IBP, MBR, mDRC, PBR, pk18, PKBS
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-18565 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSPO Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Tissue-based quantitative proteome analysis of human hepatocellular carcinoma using tandem mass tags.
Megger DA, Rosowski K, Ahrens M, Bracht T, Eisenacher M, Schlaak JF, Weber F, Hoffmann AC, Meyer HE, Baba HA, Sitek B
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 2017 Mar;22(2):113-122
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 2017 Mar;22(2):113-122
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TSPO using TSPO Polyclonal Antibody (Product # PA5-18565) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TSPO using TSPO Polyclonal Antibody (Product # PA5-18565) in paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPO in human colon. Samples were incubated with TSPO polyclonal antibody (Product # PA5-18565) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPO in human kidney. Samples were incubated with TSPO polyclonal antibody (Product # PA5-18565) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of TSPO in MCF7 cells using a polyclonal antibody (Product #PA5-18565). MCF7 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of TSPO in MCF7 cells using a polyclonal antibody (Product #PA5-18565). MCF7 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
